Behavior is the easiest way. Males are not attentive to the queen nor return to the hive. A male in mating condition has enlarged eyes. Which means you will need to compare the mating male with other bees, both male and female.
http://www.infovisual.info/02/photo/Bumb鈥?/a>
http://www.colostate.edu/Depts/Entomolog鈥?/a>How to tell from a female and a male bumble bee?
males also lack pollen carrying equipment and are never seen with lumps of pollen in the corbicula on the legs.How to tell from a female and a male bumble bee?
the shape of the stinger
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